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| Registros recuperados: 68 | |
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| Becerra V,Viviana; Paredes C,Mario; Debouck,Daniel. |
| The Chilean common bean (Phaseolus vulgaris L.) belongs to the cultivated race Chile and its origin is presumably Andean. The objective of this study was to identify the origin of a group of Chilean accessions based on their genetic relationship with wild material from the Mesoamerican and Andean common bean gene pool. To achieve this objective, universal primers of chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were used to detect polymorphism using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). Thirty-two genotypes were analyzed, including wild material from Mexico, Ecuador, Peru, Bolivia, and Argentina, as well as Chilean cultivated genotypes belonging to endemic Chilean accession types (Tórtola, Coscorrón, and... |
| Tipo: Journal article |
Palavras-chave: Chilean common bean germplasm; Chloroplast DNA; Mitochondrial DNA; PCR-RFLP. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100001 |
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| AGUIRRE-PABÓN,J.C; OROZCO BERDUGO,G. Jr; NARVÁEZ BARANDICA,J.C. |
| The tiger shrimp (Penaeus monodon) is an Indo-Pacific species. Its global production between 1970 and 1980 exceeded all other shrimp species, which favored its introduction and cultivation outside its natural range in several countries of Africa, Europe, USA and South America. It is currently found in the coast of the Atlantic Ocean (Mexico, United States, Puerto Rico, Brazil, Guyana, Venezuela and Colombia). Despite the risk involved, no studies have been conducted to evaluate their impact as a possible invasive species and their genetic condition. This study evaluated the genetic status and population origin of P. monodon in the northernmost Colombian Caribbean, analyzing the mitochondrial DNA control region (mtDNA-CR). 16 individuals were randomly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biological invasion; Caribbean Sea; Founder effect; Genetic relationships; Mitochondrial DNA. |
| Ano: 2015 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000100013 |
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| Twerdochlib,Adriana L.; Bonna,Ana C. Dalla; Leite,Selene S.; Chitolina,Rodrigo F.; Westphal,Betina; Navarro-Silva,Mario A.. |
| Genetic variability of a population of Aedes aegypti from Paraná, Brazil, using the mitochondrial ND4 gene. To analyze the genetic variability of populations of Aedes aegypti, 156 samples were collected from 10 municipalities in the state of Paraná, Brazil. A 311 base pairs (bp) region of the NADH dehydrogenase subunit 4 (ND4) mitochondrial gene was examined. An analysis of this fragment identified eight distinct haplotypes. The mean genetic diversity was high (h = 0.702; p = 0.01556). AMOVA analysis indicated that most of the variation (67%) occurred within populations and the F ST value (0.32996) was highly significant. F ST values were significant in most comparisons among cities. The isolation by distance was not significant (r = -0.1216 and p = 0,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aedes aegypti; Genetic variability; Mitochondrial DNA; Genetic population; State of Paraná. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262012000200013 |
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| Aguiar,Jonas; Schneider,Horacio; Gomes,Fátima; Carneiro,Jeferson; Santos,Simôni; Rodrigues,Luis R.; Sampaio,Iracilda. |
| The tambaqui, Colossoma macropomum, is the most popular fish species used for aquaculture in Brazil but there is no study comparing genetic variation among native and farmed populations of this species. In the present study, we analyzed DNA sequences of the mitochondrial DNA to evaluate the genetic diversity among two wild populations, a fry-producing breeding stock, and a sample of fish farm stocks, all from the region of Santarém, in the west of the Brazilian state of Pará. Similar levels of genetic diversity were found in all the samples and surprisingly the breeding stock showed expressive representation of the genetic diversity registered on wild populations. These results contrast considerably with those of the previous study of farmed stocks in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tambaqui; Mitochondrial DNA; Genetic variability; Aquaculture. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000401439 |
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| Wetzer, R.. |
| Carefully collected molecular data and rigorous analyses are revolutionizing today’s phylogenetic studies. Although molecular data have been used to estimate various invertebrate phylogenies lor more than a decade, this study is the first survey of different regions of mitochondrial DNA in isopod crustaceans assessing sequence divergence and hence the usefulness of these regions to infer phylogeny at different hierarchical levels. 1 evaluate three loci from the mitochondrial genome (two ribosomal RNAs (12S, 16S) and one protein-coding (COI)) for their appropriateness in inferring isopod phylogeny at the suborder level and below. The patterns are similar for all three loci with the most speciose suborders of isopods also having the most divergent... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Mitochondrial DNA; Isopod; Crustacea; Molecular; 12S rRNA; 16S rRNA; COI. |
| Ano: 2001 |
URL: http://www.repository.naturalis.nl/record/534397 |
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| Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea. |
| Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001 |
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| Briñez, Boris; Universidade Estadual de Maringá; Júlio Júnior, Horacio Ferreira; Universidade Estadual de Maringá; Prioli, Sonia Maria Alves Pinto; Universidade Estadual de Maringá; Maniglia, Thiago Cintra; Núcleo de Pesquisas em Limnologia, Ictiologia e Aqüicultura (Nupelia), Universidade Estadual de Maringá; Prioli, Alberto José; Universidade Estadual de Maringá. |
| Espécies de tucunaré foramn introduzidas em inúmeras bacias hidrográficas da América do Sul e em outras regiões do planeta, principalmente devido as suas características esportivas, de peixe lutador. Revisão recente das espécies do gênero Cichla mostraram que as espécies que foram introduzidas nos reservatórios das regiões Sul, Sudeste e Nordeste, são duas espécies novas, descritas como Cichla kelberi (tucunaré amarelo) e Cichla piquiti (tucunaré azul) identificadas erroneamente como C. monoculus e C. ocellaris. Com o objetivo de identificar as populações de Cichla presentes no Rio Paranapanema e Paraná, foram sequenciadas um total de 323 pares de bases (pb) da região controle (MtDNA) obtidas de oitenta e quatro indivíduos de Cichla em cinco localidades... |
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Palavras-chave: 2.02.02.00-8 species introduction; C. monoculus; Mitochondrial DNA; Peacock bass; Paraná river Genética Species introduction C.monoculus MtDNA control region Peacock bass Upper Paraná River Tucunaré. Introdução de espécies C.monoculus região controle (MtDNA) Tucunaré Genética. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/10684 |
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| Benítez Alemán, Héctor Eduardo. |
| La identificación precisa de heces de carnívoros silvestres es importante para describir sus hábitos alimenticios, sobre todo en aquellas especies elusivas o en riesgo. Sin embargo Identificar las heces de especies de carnívoros simpátricos no es una tarea sencilla. Por ello, las técnicas moleculares, son herramientas que brindan mayor precisión en la identificación de heces. El análisis de hábitos alimenticios aporta información valiosa para entender la estructura poblacional de los depredadores y sus presas, así como su importancia ecológica para generar estrategias de manejo y conservación de carnívoros y sus presas. Los objetivos de este estudio fueron identificar las heces de carnívoros extrayendo ADN mitocondrial de estas y amplificando un fragmento... |
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Palavras-chave: ADN mitocrondrial; Citocromo b; PCR-RFLP; Hábitos alimenticios; Carnívoros; Mitochondrial DNA; Cytochrome b; Food habits; Carnivores; Ganadería; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2291 |
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| Prioli,Sônia M.A.P.; Prioli,Alberto J.; Júlio Jr.,Horácio F.; Pavanelli,Carla S.; Oliveira,Alessandra V. de; Carrer,Helaine; Carraro,Dirce M.; Prioli,Laudenir M.. |
| Astyanax fishes are among the most important food-web components of South America rivers. In the Iguaçu River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Iguaçu Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti & Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Iguaçu... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax; Fish; D-loop; Control region; Mitochondrial DNA; RAPD. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400011 |
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| Sodré,Davidson; Rodrigues-Filho,Luis F.S.; Souza,Rosália F.C.; Rêgo,Péricles S.; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo. |
| Carcharhinus limbatus has a cosmopolitan distribution and marked genetic structuring, mainly because of its philopatric behavior. However, analysis of this structuring has not previously included South American populations. In the present study, we analyzed a sample of adult individuals collected on the northern coast of Brazil and compared the sequences of the mitochondrial control region with those of populations already genotyped. Relatively high haplotype diversity (12 haplotypes, genetic diversity of 0.796) was observed, similar to that in other populations but with a much larger number of private alleles. In contrast to populations studied previously, which were represented by neonates, the pronounced allelic variability found in the South American... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carcharhinus limbatus; Control region; Fishery stocks; Mitochondrial DNA; Population genetics. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500008 |
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| Gomes,Fátima; Schneider,Horacio; Barros,Claudene; Sampaio,Dioniso; Hashimoto,Diogo; Porto-Foresti,Fábio; Sampaio,Iracilda. |
| Tambaqui (Colossoma macropomum) is the fish species most commonly raised in the Brazilian fish farms. The species is highly adaptable to captive conditions, and is both fast-growing and relatively fecund. In recent years, artificial breeding has produced hybrids with Characiform species, known as "Tambacu" and "Tambatinga". Identifying hybrids is a difficult process, given their morphological similarities with the parent species. This study presents an innovative molecular approach to the identification of hybrids based primarily on Multiplex PCR of a nuclear gene (α-Tropomyosin), which was tested on 93 specimens obtained from fish farms in northern Brazil. The sequencing of a 505-bp fragment of the Control Region (CR) permitted the identification of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pisciculture; Multiplex PCR; Mitochondrial DNA; Genetic bottleneck. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200020 |
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| Aguirre-Obando,Oscar A.; Bona,Ana C. Dalla; Duque L.,Jonny E.; Navarro-Silva,Mário A.. |
| Mosquito control prevails as the most efficient method to protect humans from the dengue virus, despite recent efforts to find a vaccine for this disease. We evaluated insecticide resistance and genetic variability in natural populations of Aedes aegypti (Linnaeus, 1762) from Colombia. This is the first Colombian study examining kdr mutations and population structure. Bioassays with larvae of three mosquito populations (Armenia, Calarcá and Montenegro) were performed according to the World Health Organization (WHO) guidelines, using Temephos. For the analysis of the Val1016Ile mutation and genetic diversity, we sampled recently-emerged adults from four mosquito populations (Armenia, Calarcá, Montenegro and Barcelona). Following the WHO protocol, bioassays... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioassays; Gene flow; Knockdown resistance; Mitochondrial DNA; ND4 gene. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702015000100014 |
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| TAKAHASI, Shoko; MIYAHARA, Kazuro; ISHIKAWA, Hirosi; ISHIGURO, Naotaka; SUZUKI, Mitsuyoshi; 宮原, 和郎; 鈴木, 三義. |
| To estimate the maternal effects of dog breeds using mitochondrial DNA(mtDNA) haplotypes in the dogs with several clinical disorders, 600 base pairs of mtDNA D-loop region were amplified from 365 dogs and were determined for mtDNA sequences. The diversity of the 600-bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV. Lineage analysis using the mtDNA haplotype indicated that each dog breed genetically comprises one or a few mtDNA haplotypes. When the relationship between genetic background and occurrences of clinical diseases was estimated, canine lineage analysis using mtDNA haplotype revealed that the disorders distributed in the dominant mtDNA haplotypes of... |
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Palavras-chave: D-loop; Haplotype lineage analysis; Mitochondrial DNA. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4066 |
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| Rodrigues,Rosa; Schneider,Horacio; Santos,Simôni; Vallinoto,Marcelo; Sain-Paul,Ulrich; Sampaio,Iracilda. |
| The acoupa weakfish (Cynoscion acoupa - Sciaenidae) is a marine species of croaker with estuarine-dependent behavior, found in the western Atlantic from Panama to Argentina. It is one of the most exploited food fish on the northern coast of Brazil. In this study, DNA sequences were determined from the entire control region (D-loop) of the mitochondrial genome of 297 individuals collected during seven different months between December 2003 and August 2005 on the northern coast of Brazil (Amapá and Pará). Genetic variability expressed by haplotype (h = 0,892) and nucleotide (pi = 0,003) diversities were low compared to other heavily exploited marine fish species from the western Atlantic and eastern Asia. AMOVA depicted a lack of genetic structuring among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cynoscion acoupa; Acoupa weakfish; Sciaenidae; Low genetic diversity; Mitochondrial DNA; D-loop. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300015 |
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| Boudry, Pierre; Huvet, Arnaud. |
| The taxonomic status of Crassostrea angulata and Crassostrea gigas has long been a matter of controversy. Morphological and physiological similarities, as well as homogeneity in allelic frequencies on allozymes between the populations of the two taxa, lead most authors to suggest to regroup of the two within the same species. European and Asian populations of C. gigas and C. angulata have been studied using microsatellite and mitochondrial DNA markers. The analysis of genetic distances and the distribution of allelic and haplotype frequencies revealed a differentiation between the populations of C. gigas and C. angulata. The data allowed the construction of Neighbor-joining trees for each of the two types of markers. Similar topologies appeared with data... |
| Tipo: Text |
Palavras-chave: Mitochondrial DNA; Nuclear DNA; Phylogeography; Crassostrea angulata; Crassostrea gigas; Genetic; Oyster. |
| Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/acte-3434.pdf |
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| Aral,Cenk; Akkiprik,Mustafa; Kaya,Handan; Ataizi-Çelikel,Çigdem; Çaglayan,Sinan; Özisik,Gökhan; Baloglu,Hüseyin; Özer,Ayse. |
| Recently, efforts have been focused on mitochondrial DNA changes and their relation to human cancers. Among them, a 4977 bp deletion of mitochondrial DNA, named "common deletion", has been investigated in several types of tumors, with inconsistent results. In this study, we investigated the presence of the common deletion in tissues from 25 breast, 25 colorectal and 50 thyroid tumors and in the adjacent healthy tissues from Turkish patients. Samples from healthy volunteers were also evaluated for comparison. Two PCR-based methods were used for the detection of the common deletion. First, two pairs of primers were used to amplify wild-type and deleted mtDNA. Then, a highly sensitive nested-PCR was performed, to determine low amounts of deleted genomes. By... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mitochondrial DNA; Common deletion; Cancer. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100001 |
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| Yunis,Juan J.; Yunis,Emilio J.. |
| The frequencies of four mitochondrial Native American DNA haplogroups were determined in 1526 unrelated individuals from 11 Departments of Colombia and compared to the frequencies previously obtained for Amerindian and Afro-Colombian populations. Amerindian mtDNA haplogroups ranged from 74% to 97%. The lowest frequencies were found in Departments on the Caribbean coast and in the Pacific region, where the frequency of Afro-Colombians is higher, while the highest mtDNA Amerindian haplogroup frequencies were found in Departments that historically have a strong Amerindian heritage. Interestingly, all four mtDNA haplogroups were found in all Departments, in contrast to the complete absence of haplogroup D and high frequencies of haplogroup A in Amerindian... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Admixture; Colombia; Haplogroup; Mestizo; Mitochondrial DNA. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300005 |
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| Registros recuperados: 68 | |
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